Medical Biotechnology

Stem Cell and Cryo Technology & Bioprocessing and Bioanalytics

Mobile epidemiological laboratories including BSL2 area for site-flexible performance of medical examinations and anthropometric measurements, collection of human samples, sample characterization, sample processing, clinical chemical analysis and LIN-based cryopreservation

Safety class S2 and S3 laboratories with lock areas for microbiological, molecular biological, cell biological and virological work

• cell culture robots (e. g. Tecan Freedom, TAP)
• various transmitted and reflected light microscopes with phase and differential
• interference contrast, fluorescence, manipulation and documentation unit and incubation chamber (e. g. Nikon, EVOS)
• transmitted light and fluorescence microscope with z-axis adjustable sample
• stage for 3D imaging of biological samples (Zeiss AxioObserver Z1)
• confocal microscope equipped with white light laser and continuous wave laser of wavelength 405 nm (Leica-TCS-SP8-X)
• live cell imaging units (e. g. Nikon BioStation-IM)
• HPLC system (Agilent 1260 Infinity Series)
• mass spectrometer (Q Exactive FOCUS)
• asymmetric field flow fractionation system with DLS and MALS detector
• flow cytometer including sorting unit (e.g. FACSAria, FACSCanto BD
• spectrophotometer for absorbance, fluorescence and luminescence measurements in microtiter plates
• TER impedance measurement systems (cellZscope®, Ussing chamber®) for transport studies and barrier function analysis of liquid-liquid interfaces
• Franz Cell System® (air-liquid interface model, e.g. skin barrier)
• aerosol exposure system VITROCELL® Cloud (air-liquid interface model, e. g. lung barrier)
• bioprinter for tissue engineering (Rokit invivo) and µContact printing (GeSiM µCP)
• various pipetting robots (GeSim Nanoplotter, Integra ViaFlo)
• ZetaSizer ® Nano
• gel electrophoresis units for DNA, RNA and proteins with documentation units (e. g. Bioanalyzer, Agilent)
• Western blot unit (BioRad)
• real time PCR system (QuantStudio 7, Applied Biosystems) and NanoDrop 2000 (Peqlab)
• freezing microtome

Laboratory for automation of stem cell processes and material characterization (Würzburg)

• Suspension bioreactors (OLS, CERO)
• multi-electrode array and impedance measurement system for electro-physiological studies (Axion Biosystems, Maestro Pro)
• automated cell counters (Chemometec, Nucleocounter)
• osmometers (Gonotec, Osmomat 3000)
• refractometers (Kern Optics, Abbe)
• spectrophotometer (Thermo ScientificTM NanodropTM One) for determination of DNA concentration
• rheometer for viscosity measurements (Anton Paar, MCR102)
• nano-indenter for the investigation of mechanical material properties (Optics11 Life, Pavone)
• plasma pen for surface modification (neoplas GmbH, kINPen)
• 3D printer (ROKIT, 3D Bioprinter INVIVO)

Optics laboratory for the operation and development of optical measurement technology

• ultra-short pulsed Ti:sapphire laser, various other pulsed and cw laser sources
• confocal Raman microscopy, Raman microspectrometry
• multiphoton laser scanning microscope (Miltenyi TrimScope Matrix)
• UV/Vis/NIR spectrometer (Perkin Elmer Lambda 950)
• fluorescence spectrometer (Hitachi FL4500)
• dynamic differential calorimetry (Perkin Elmer DSC8500), operable from -180°C to 600°C

Cryolab for preservation of biological samples in conventional and vitrification-based methods

• Askion C-line automated biobank and manual nitrogen-based storage tanks
• cryogenic workbenches for work below 80°C (Askion)
• controlled freezing equipment (e. g. SyLab, ViaFreeze Asysmtote)
• temperature control units for controlled thawing (e. g. Thawstar BioCision)
• freeze dryer (Martin Christ)
• programmable cryogenic chambers for microscopy (Cryostage Linkam)

Microsystems technology

• coating/microstructuring
  • photolithography
  • dry etching equipment (RIE) and wet chemical etching
  • coating (sputtering (metals, piezoelectric coatings), PECVD: silicon dioxide, silicon nitride, deposition of Parylene C)
  • imidization of polyimide
• wafer saw
• 3D printer
• silicone molding
  
• packaging and interconnection technologies (bonding, encapsulation, wire bonding)
• printing technologies
  • screen printing (thick film pastes on ceramics, graphene-based ink on polymer films)
  • roll-to-roll printing on polymer films (graphene-based ink, protein inks)
  • inkjet printing on polymer films and glass (carbon and metal inks)
• 3D CAD (SolidWorks)